Transcriptional activation and sensing properties
نویسندگان
چکیده
Tag der mündlichen Prüfung am: The voyage of discovery is not in seeking new landscapes but in having new eyes-Marcel Proust ABBREVIATIONS 6 Abbreviations ADAM 1-aminoadamantam ADP Adenosine diphosphate ATP Adenosine triphosphate AHT Anhydrotetracycline Amp r Resistance towards ampicilin aphA3, Kan r Resistance towards kanamycin APS Ammoniumperoxodisulfate bp Base pairs BCA Bicinchoninic acid cat, Cml r Resistance towards cholramphenicol CIAP Calf intestine alkaline phosphatase DEPC Diethyl Pyrocarbonat DIG Digoxygenin DTT Dithiothreitol EDTA Ethylendiaminetetraacetic acid Erm, Ery r Resistance towards erythromycin et. al. et alii (" and others ") FMOC 9-Fluorenyloxycarbonylchlorid g Gravitational acceleration (9.81m/s) HPLC High-performance liquid chromatography IPTG Isopropyl-1-thio-β-D-galactosid kDa kilo Dalton LB Luria-Bertani MM Minimal medium MOPS 3-[N-morpholino] propanesulfonic acid OD 578 Optical densitiy at 578nm PAGE Polyacrylamide gel electrophoresis PCR Polymerase chain reaction PNPG para-Nitrophenyl-α-D-Glucopyranoside Rpm Rotations per minute RT Room temperature SDS Sodium dodecylsulphate TAE Tris acetate EDTA TEMED N,N,N',N'-tetramethyl-ethylendiamine Tris 2-aminohydrohymethylpropane-1,3-diol v/v Volume per volume w/v Weight per volume
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